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. Author manuscript; available in PMC: 2013 Jul 28.
Published in final edited form as: Methods Mol Biol. 2012;832:349–362. doi: 10.1007/978-1-61779-474-2_24
Stock 100 ml
50 mM HEPES, pH 7.5 1 M 5 ml
150 mM NaCl 4 M 3.75 ml
1 mM MgCl2 1 M 100 µl
10% glycerol 75% 13.3 ml
0.2 mM CuCl2 0.2 M 100 µl
Note: the same buffer without CuCl2 should also be prepared.