Skip to main content
. Author manuscript; available in PMC: 2013 Jul 28.
Published in final edited form as: Nat Methods. 2012 Jan 30;9(2):117–118. doi: 10.1038/nmeth.1865

Figure 1.

Figure 1

Improvements to Mos1-based genome manipulation. (a) A plasmid expressing transposase under the indicated promoters was coinjected with low DNA concentration (32.5 ng/ul) of a 4.4 kb transgene. The plot shows insertion frequency into the ttTi5605 locus. (b) The plot shows the frequency of a 5 kb targeted deletion of dpy-13. Pglh-2 data from Frøkjær-Jensen et al (2010)2) using the indicated selection markers (see Supplementary Methods for discussion). (c) Insertion frequency with higher total DNA concentrations (~100 ng/ul) and in the presence of the negative selection marker peel-1. Error bars, 95 % confidence intervals, significance was determined with Fischer’s exact test