Figure 3.
A3R agonism attenuated neutrophil chemotaxis in vitro. Neutrophils from WT mice were added to the upper wells of a trans-well migration assay, incubated with Cl-IB-MECA (MECA, 1 µM) or vehicle, and then exposed to media or media with 10% fetal bovine serum (FBS) in the lower wells. After incubating for 4 hrs at 37°C, trans-migrated cells were quantified and presented as a chemotaxis index. *p<0.05 vs. media + vehicle, #p<0.05 vs. 10% FBS + vehicle (n=8/group).