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. 2013 Jun 14;27(8):1283–1294. doi: 10.1210/me.2012-1405

Figure 5.

Figure 5.

Kisspeptin Induced Egr-1 through SRF Sites and cFos through Homeobox and STAT Sites. LβT2 cells were transiently transfected with a Kiss1R expression vector and the indicated luciferase reporter. Cells were treated for 12 hours with vehicle (DMSO for kisspeptin and 0.1% BSA for GnRH), 100 nM kisspeptin, or 10 nM GnRH as indicated and subjected to luciferase assay. Data were normalized to vehicle-treated control. A, Diagram of the proximal Egr-1 promoter containing SRF, CRE, and ETS sites. B, Diagram of the proximal cFos promoter containing SRF, AP-1, STAT, and hox sites. C and E, Mutations in the Egr-1 promoter were transfected into LβT2 cells, which were treated with vehicle, 100 nM kisspeptin, or 10 nM GnRH. Kisspeptin and GnRH induction for all mutants were compared across to wild-type. *, P < .05 by Student's t test; ***, P < .001 by 1-way ANOVA. D and F, Mutations in the cFos promoter were transfected into LβT2 cells, which were treated with vehicle, 100 nM kisspeptin, or 10 nM GnRH. Kisspeptin and GnRH induction for all mutants were compared across to wild-type. **, P < .01; ***, P < .001 by 1-way ANOVA. mut., mutation.