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. 2013 Jun 14;27(8):1283–1294. doi: 10.1210/me.2012-1405

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Copyright © 2013 by The Endocrine Society

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Figure 7. — Kisspeptin (Kiss) Induction of Gonadotrope Genes and Regulation of Pituitary Kiss1r Expression in Vivo. Primary pituitary cells from male mice (10 animals/group) were harvested and treated for 6 hours with 100 nM kisspeptin or vehicle. qPCR was performed for LHβ (A) and FSHβ (B). Data are normalized to TBP. *, P < .05; and **, P < .01 compared with control by Student's t test. C, Pituitaries were harvested from OVX and OVX+E females for both am and pm groups (4–9 animals/group). qPCR was performed for Kiss1r, and data are normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). ***, P < .001 by 2-way ANOVA. D, Serum LH was measured from am and pm OVX+E females. *, P < .05 by Student's t test. TBP, TATA-binding protein.