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. 2013 Jul 28;19(28):4495–4503. doi: 10.3748/wjg.v19.i28.4495

Figure 2.

Figure 2

Silencing of basic transcription factor 3 using small interfering RNA. A: Transfection rates of small interfering RNA (siRNA)-normal control (NC)/siRNA-basic transcription factor 3 (BTF3)-green fluorescent protein (GFP) vectors in human SGC7901 cells, estimated by bright field and fluorescence microscope; B: Bar charts represent quantitative real-time polymerase chain reaction data of BTF3 mRNA levels in control and BTF3-siRNA transfected SGC7901 cells (bP < 0.01 between groups, unpaired t test); C: Immuno-blotting analysis using BTF3-specific antibodies to detect BTF3 protein expressions in control and BTF3-siRNA transfected SGC7901 cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as internal control.