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Luciferase (lux) expression after bactofection of cystic fibrosis tracheal epithelial (CFTE29o—) cells with invasive and noninvasive E. coli carrying the modified pCIKLux (pCIKLux-mod). CFTE29o— cells were infected at MOI 50 with invasive E. coli BM4570 and non-invasive E. coli BM2710 carrying the pCIKLux-mod (pCIKLux-mod). After 48 h of infection cells were harvested and lux activity assayed. Data are expressed as mean ± s.e.m. (n = 6).
