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Sequence alignment of wild type enzymes by Clustal W. (Larkin et al., 2007), sequence identities in amino acid sequences: AtSPDS1 to DsSPDS1 82%, AtSPDS1 to DsPMT 60%, DsSPDS1 to DsPMT 67%. Changes by site-directed mutagenesis in coenzyme binding site: gray, changes in putrescine binding site: black; Gonnet-Matrix; *single, fully conserved residue; : conservation between groups of strongly similar properties;. conservation between groups of weakly similar properties.
