Table 1.

Primers for mutagenesis of DsPMT, DsSPDS1 and AtSPDS1, mutated triplets are underlined.

Exchanged amino acids	Mutagenesis primers 5′–3′
DsPMT
H108L	Fwd	GGATGGAGCAATTCAACTCACAGAGAATGGTGGATTTC
	Rev	GAAATCCACCATTCTCTGTGAGTTGAATTGCTCCATCC
DsSPDS1
L70H	Fwd	GGTGTGATACAACACACAGAGCGGGATG
	Rev	CATCCCGCTCTGTGTGTTGTATCACACC
Q79T	Fwd	GATGAATGTGCTTACACAGAAATGATCACTCATCTCC
	Rev	AGATGAGTGATCATTTCTGTGTAAGCACATTCATCCC
D103I	Fwd	GTTATTGGAGGAGGTATTGGTGGTGTCTTGCGTG
	Rev	CACGCAAGACACCACCAATACCTCCTCCAATAAC
V106T (with D103I)	Fwd	GGAGGTATTGGTGGTACCTTGCGTGAGGTGTCTCG
	Rev	CGAGACACCTCACGCAAGGTACCACCAATACCTCC
AtSPDS1
L98H	Fwd	GTTTTGGATGGAGTAATCCAACATACGGAGAGAGATG
	Rev	CATCTCTCTCCGTATGTTGGATTACTCCATCCAAAAC
Q107T	Fwd	GAGAGAGATGAATGTGCTTATACGGAAATGATCACTCATCTTCC
	Rev	GGAAGATGAGTGATCATTTCCGTATAAGCACATTCATCTCTCTC
D131I	Fwd	GTCATTGGAGGAGGAATTGGAGGTGTCCTGCGG
	Rev	CCGCAGGACACCTCCAATTCCTCCTCCAATGAC
V134T (with D131I)	Fwd	GGAGGAATTGGAGGTACCCTGCGGGAAGTTGC
	Rev	GCAACTTCCCGCAGGGTACCTCCAATTCCTCC
D204A	Fwd	GTTATTGTTGACTCTTCAGCTCCAATCGGTCCTG
	Rev	CAGGACCGATTGGAGCTGAAGAGTCAACAATAAC
D204K	Fwd	GATGCAGTTATTGTTGACTCTTCAAAACCAATCGGTCCTGC
	Rev	GCAGGACCGATTGGTTTTGAAGAGTCAACAATAACTGCATC
E236A	Fwd	GTGTGCACTCAAGCTGCGAGCTTGTGGCTTCAC
	Rev	GTGAAGCCACAAGCTCGCAGCTTGAGTGCACAC
Y270A	Fwd	CAGCGTTCCAACAGCCCCCAGTGGGGTCAT
	Rev	ATGACCCCACTGGGGGCTGTTGGAACGCTG