Fig. 9.

Effect of ATP on SLC26A6 (A6) protein expression. A: representative Western blot of total and surface biotinylated SLC26A6. C2 cells were preincubated with vehicle (CON) or 100 μM ATP for 30 min, and SLC26A6 protein expression was evaluated in cell lysate [total (50 μg protein/lane)] and after streptavidin precipitation of surface biotinylated proteins from 2,000 μg of initial cell lysate (surface). Anti-GAPDH antibody was used to verify equal loading of protein in each lane. B and C: densitometry of immunoblot results. Western blot band density was quantified using ImageJ software. Values (means ± SE for 5 independent experiments for cells grown on plastic supports and 6 independent experiments for cells grown on Transwell inserts) represent total SLC26A6 abundance relative to GAPDH and surface biotinylated SLC26A6 relative to total SLC26A6 and are presented as a percentage of the respective control value. *P < 0.003 vs. CON (by paired t-test).