Fig. 7.

Inversin depletion does not prevent contractile ring assembly or proper mitotic spindle formation. A: HeLa cells transfected with either myosin light chain kinase GFP, ceRhoA:GFP, or anillin:GFP fixed with 3% paraformaldehyde to preserve the fluorescent tag and stained with anti-β-tubulin antibody and Hoescht 33342 DNA stain. Note that all three components of the contractile ring still accumulated normally at the equatorial plane and the ingressing furrow. B: measurement of spindle length in control RNAi-treated and inversin-depleted cells from fixed HeLa cells. Averages of at least 40 individual measurements are shown in the graph. There was no significant difference in spindle length between control RNAi-treated cells and inversin-depleted cells (P = 0.785).