Table 1.

Colocalization of filamin, CFTR, and RACK1 in Calu-3 cells

Protein1	Protein2	Rpearson	Roverlap	M1	M2	ICQ	n (cells)
Filamin	CFTR	0.61 ± 0.04	0.71 ± 0.06	0.28 ± 0.06	0.57 ± 0.07	0.35 ± 0.05	12 (130)
Filamin	RACK1	0.70 ± 0.06	0.79 ± 0.04	0.39 ± 0.06	0.57 ± 0.04	0.37 ± 0.02	6 (47)
CFTR	RACK1	0.50 ± 0.03	0.60 ± 0.03	0.24 ± 0.04	0.35 ± 0.04	0.29 ± 0.04	8 (80)

All values shown are means ± SE. CFTR, cystic fibrosis transmembrane regulator; RACK1, receptor for activated C kinase 1; R_pearson, Pearson's correlation coefficient (−1; negative correlation, e.g., exclusion; 0: no correlation; 1: positive correlation); R_overlap, overlap correlation coefficient, similar to Pearson's coefficient but without subtraction of the mean intensity values in each channel (0: negative correlation; 1: positive correlation); M1 and M2: Manders coefficients, quantifying the fractional intensity overlap of the first protein's immunofluorescence signal with that of the second protein (M1) and vice versa (M2); ICQ, intensity correlation quotient, as defined by Li et al. (Ref. 6; −0.5: exclusion; 0: random with uncorrelated intensities; 0.5: colocalization with completely correlated intensities between the 2 channels); n (cells): n, total number of imaged fields; cells, total number of cells in these fields