Fig. 4.

Relevance to myocardial tissue. A: LV Cr levels in mice overexpressing the Cr transporter (CrT-OE) were significantly higher than wild type (mean 124 nmol/mg protein vs. 74 nmol/mg, respectively; P = 0.0012), whereas Cr was confirmed to be undetectable in hearts from guanidinoacetate methyltransferase knockout (GAMT^−/−) mice. B: GAMT^−/− had lower Txnip transcript levels than wild-type littermates (60 ± 7; P = 0.021). In contrast, CrT-OE showed higher levels of Txnip transcript compared with the wild type (158 ± 15; P = 0.0397). C: protein expression of Txnip was not statistically different in GAMT^−/− compared with the wild type, whereas CrT-OE had a 29% increase in protein (P = 0.012). Wild-type n = 10; GAMT^−/− n = 5; CrT-OE n = 8. Statistical symbols on graphs correspond to nonsignificant (ns) P > 0.05, *P < 0.05, and **P < 0.005.