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. Author manuscript; available in PMC: 2013 Jul 29.
Published in final edited form as: J Immunol. 2010 Feb 24;184(7):3433–3441. doi: 10.4049/jimmunol.0904028

FIGURE 3.

FIGURE 3

Expression of Helios during peripheral T cell development. A, Splenocytes from two individual mice at the indicated ages were analyzed by flow cytometry for Helios and Foxp3 expression. Plots shown are gated on CD4+ cells. B, Splenocytes from two individual adult or 4-d-old mice were analyzed by flow cytometry for CD25 and CD44 expression. Plots shown are gated on CD4+ cells. C, Splenocytes from two individual mice at the indicated ages were analyzed by flow cytometry for CD25 and CD44 expression. Plots shown are gated on CD4+ Foxp3+Helios− cells. Mice of different ages were analyzed on different days but were always compared with an adult mouse. Shown are representative plots of one mouse from at least two independent experiments.