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FOXP3+Helios− T cells are enriched in cytokine-producing cells. PBLs from healthy adults were activated for 5 h with PMA/ionomycin, gated on CD4+Foxp3+ cells, and analyzed for Helios and cytokine expression. Panels on the left show a representative plot of Helios versus the indicated cytokine, whereas panels on the right show cumulative data from five healthy adults.
