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. Author manuscript; available in PMC: 2013 Jul 29.
Published in final edited form as: J Immunol. 2010 Dec 10;186(2):838–847. doi: 10.4049/jimmunol.1001735

Figure 7. Chronic treatment of mice with αGC or IL-18+IL-12 ablates peripheral iNKT cells followed by a protracted repopulation of the liver that requires the thymus.

Figure 7

(A) Mice were treated chronically with αGC on days 0, 2, 4, 8, 10, and VC and IL-18+IL-12 on days 0–4 and 8–10 (chronic treatment regimen). Fold change in iNKT cells relative to VC at indicated time points was determined by qPCR and FCA as in figure 1 using 6 individual animals from 2 independent experiments ± SEM. (B) Liver repopulation of iNKT cell kinetics in sham and thymectomized mice treated chronically, as in A, with VC, αGC and IL-18+IL-12. Liver leukocytes were isolated from the liver at indicated days and the number of iNKT cells was determined by FCA as described in figure 1. Day 80 represents 9 individual animals from 3 independent experiments ± standard error. All other days represent 6–9 animals from 2–3 independent experiments ± SEM.