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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: J Immunol. 2013 May 17;190(12):6626–6634. doi: 10.4049/jimmunol.1203330

Figure 2. H. pylori induces apoptotic cell death in primary human gastric epithelial cells.

Figure 2

(a, b) Gastric epithelial cells were cultured for 3 days on collagen-coated plates and then treated with H. pylori (2×107/mL), camptothecin (CMP, 5 µM) or medium alone. Apoptosis was determined by flow cytometric analysis of Annexin-V-FITC/propidiumiodide (PI)-stained cells after 6–8 h. (a) Representative data and (b) cumulative data from 10 (untreated, H. pylori) and 5 (CMP) experiments; mean ± SEM. (c) Microscopic analysis of ZO-1-Cy3/phalloidin-FITC/DAPI-stained epithelial monolayers after 24 h exposure to H. pylori or camptothecin. Arrows indicate areas with altered ZO-1 immunolocalization. Results are from a representative experiment (n=4).