Figure 4. H2009 conditioned medium increases MOR expression in benign Beas2B bronchoepithelial cells.

Beas2B epithelial cells incubated with H2009 conditioned medium for 48h as compared to H2009 blank medium show significantly increased expression of MOR. Density units of MOR:GAPDH are expressed as mean ± SEM of 3 separate experiments. No DOR expression was observed on Beas2B cells incubated with or without H2009 conditioned medium (data not shown). X-axis shows different medium used as follows: Beas2B, Complete Beas2B culture medium BEBM with Singlequotes; H2009 Blank, H2009 culture medium without serum incubated for 48h in a T-75 flask without any cells; H2009 conditioned, H2009 medium incubated with H2009 cells for 48h. Abbreviations: GAPDH: glyceraldehyde 3-phosphate dehydrogenase; MOR: mu opioid receptor