Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Western blots revealed protein expression levels in knock-in cells and monitored the efficiency of affinity purification. (A) 3×FLAG tagged proteins expressed at similar levels as endogenous proteins in knock in cells. (B) Significant enrichment of target proteins and their respective complexes by affinity purification. M refers to standard protein marker, L refers to lysate, W refers to wash, E refers to elution fraction and FT refers to flow through.
