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Western blot verifications of selected known and novel APC interactors. Elutions from IP by α-FLAG/APC were loaded on SDS-PAGE followed by Western blot analyses with antisera against APC interactors, β-catenin, APC, and TPM1. M refers to standard protein marker and rep in sample name refers to biological replicates. Antisera α-tubulin and β-catenin were used as loading controls for equal loading of cell lysate for each IP experiment.
