Fig. 1.

Identification of a concanavalin A (ConA)-reactive intercellular adhesion molecule 1 (ICAM-1) glycoform on activated endothelial cells. A: human umbilical vein endothelial cells (HUVECs) were stimulated with TNF-α (10 ng/ml, 6 h) with or without pretreatment with swainsonine (swain) or kifunensine (kif). Lysates were analyzed by Western blot analysis for ICAM-1 expression. B: HUVECs were stimulated with TNF-α as above and labeled with biotinylated lectins (ConA, SNA, MAA, PHA-L, or LCA; see Table 1 for definitions) and purified against streptavidin. Whole cell lysates (WCL) and streptavidin bound surface proteins were analyzed by Western blot analysis for ICAM-1 and β-actin. Data are representative of at least 4 separate experiments.