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ConA reactive ICAM-1 is present in human atherosclerotic vessels. Coronary arteries were collected postmortem and lysed as described in materials and methods. Lysates were incubated with ConA-Sepharose to enrich for high-mannose N-glycan containing proteins. Total lysate and ConA bound proteins were analyzed by Coomassie staining (A) and Western blot analysis for ICAM-1 (B). Data are representative from 4 independent coronary arteries.
