Fig. 1.

Hyperoxia-exposed Nucleotide-binding domain and leucine-rich repeat PYD-containing protein 3-deficient (NLRP3^−/−) mice show attenuated lung injury. wild-type (WT) and NLRP3^−/− mice were exposed to room air or 100% O₂ for 48 h. Mice were euthanized and lung tissues were collected. A: representative hematoxylin-eosin (H&E)-stained lung tissue sections. Original magnifications: ×40 (insets, ×100). B: semiquantitative lung injury analysis by evaluation of H&E-stained lung tissue sections. Total histopathological score was expressed as summed scores for 4 independent parameters: alveolar congestion, hemorrhage, aggregation of neutrophil or leukocyte infiltration, and thickness of the alveolar wall. Results (means ± SE; n = 8 in each group) are cumulative data of 2 independent experiments. *P < 0.05.