Fig. 1.
Real-time PCR results for galectin-3 mRNA levels in normal and acidotic rabbit kidneys. Real-time PCR was performed on RNA extracted from 6 normal and acidotic rabbit kidney cortical tissues with galectin-3 SYBR Green primers as described in materials and methods. Relative quantity (RQ) was obtained from threshold cycle (Ct) values with the ΔΔCt method. GAPDH Ct values of corresponding samples were used to normalize the expression levels. Two-tailed Student t-test was performed with Microsoft Excel to obtain P values.