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. 2013 May 8;305(1):F90–F99. doi: 10.1152/ajprenal.00498.2012

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Fig. 2. — Western blot results for galectin-3 levels in total cell lysates (A) and conditioned medium (B) of low-density (LD) and high-density (HD) clone C cells. A, left: 40 μg of cell lysates extracted from HD and LD monolayers was probed with guinea pig polyclonal sera against rabbit galectin-3, and the same amount of total cell lysates was probed with actin and GAPDH antibodies. Right: densitometric values obtained from 4 independent Western blots. B, left: 30 μg of concentrated conditioned medium obtained from HD and LD cell cultures was probed with galectin-3 antibody, and in control studies the same amount of conditioned medium was probed with cyclophilin A (CypA) antibody. Right: densitometric values obtained from 4 independent Western blots.