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. 2013 May 8;305(1):F90–F99. doi: 10.1152/ajprenal.00498.2012

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Fig. 8. — Western blot of kidney tissue extract and clone C cell lysate with galectin-9 antibody (A) and OptiPrep density gradient fractions of conditioned medium incubated with recombinant galectin-9 (B). A: a goat polyclonal antibody to galectin-9 was used for Western blot of 20 μg of tissue extract from cortical, medullary, and papillary tissues of normal rabbit kidney (lanes 2–4) along with 40 μg of recombinant galectin-9 (lane 1) and 40 μg of clone C lysates (HD, lane 5; LD, lane 6) and conditioned medium (lane 7). The antibody recognized an ∼45-kDa band in lane 1 containing the recombinant galectin-3, confirming its specificity. B: anti-hensin Western blot of OptiPrep density gradient fractions of LD conditioned medium incubated with 0.7 μM (32 μg/ml) of recombinant galectin-9, showing oligomerization of hensin to fraction 7.