Fig. 2.

Chronic alcohol feeding induces increased intestinal Cyp2e1 protein in BL/6 mice. C57BL/6 mice were pair fed a complete liquid diet (detailed in materials and methods) for 8 wk containing alcohol (EtOH BL/6; 4.5% vol/vol, 1 M) or control diet with calories matched with dextrose. Cyp2e1 protein expression was analyzed in proximal colon tissue by Western blotting tissue from N = 4 mice for each condition. Representative blot for tissue from 1 mouse for either control or alcohol-fed mice. Blot was stripped and reprobed for actin as a loading control. Histogram data depicts summarized data for all mice N = 4 for each condition. *P < 0.05 vs. control.