Fig. 2.
Inhibition of tumor necrosis factor (TNF) and tumor necrosis factor receptor 1 (TNFR1) release after lipopolysaccharide (LPS) injection in ADAM17 m-KO and ADAM17 h-KO mice. Data presented are from samples collected 90 min after injection of saline or LPS (1 μg/g body wt). A: serum levels of TNF, as determined by ELISA, are significantly lower in ADAM17 h-KO and ADAM17 m-KO mice after injection of LPS compared with controls (n = 4–5). B: LPS induction of Tnf mRNA in the liver is similar between Cre-, ADAM17 m-KO, and ADAM17 h-KO mice (black, hatched, and open bars, respectively) (n = 2–5). C: serum levels of TNFR1, as determined by ELISA, are lower in ADAM17 h-KO and ADAM17 m-KO mice after injection of LPS compared with controls (n = 3–4). D: serum levels of IL-6 are significantly lower in ADAM17 h-KO mice, but not ADAM17 m-KO mice, compared with control mice after LPS injection (n = 5). ELISA data are presented as means ± SE. Real-time RT-PCR data are presented as fold change using the values from untreated Cre- controls as the denominator and are presented as means ± SE. *P < 0.05 and **P < 0.01 compared with Cre- group and normalized to 18S expression. ND, not detectable. Cre- represents control Adam17FlNeo/FlNeo mice.