Fig. 3.
Real-time PCR analysis of TNFα, IFNγ, and IL-1β mRNA expression in the proximal and distal colon of control and IL-10−/− CD4+ T cell-transferred mice maintained on the low vitamin D3 diet or switched to the high vitamin D3 diet. Data were normalized to GAPDH mRNA as an internal control and calculated using the ΔΔCt method, with control mice on the low vitamin D3 diet used as a calibrator. Values are means ± SE; n = 5 and 7 for control and colitis groups, respectively. Statistical analysis was performed with ANOVA followed by Fisher's PLSD post hoc test, with P ≤ 0.05 considered significant.