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. 2013 Jul 9;2013:574029. doi: 10.1155/2013/574029

Figure 3.

Figure 3

Analysis of RAGE expression on dendritic cells (DCs). Five-day human DCs pretreated or not with resveratrol (Resv, 50 μM) were cultured for 18 hours with or without AGE-albumin (AGE, 30 μg/mL). DCs treated with LPS (0.2 μg/mL), albumin (Alb; 30 μg/mL) and resveratrol (Resv; 50 μM) were used as controls. (a) Flow cytometric analysis of RAGE expression. AGE-albumin (AGE) induced a statistically significant upregulation of RAGE (panel (i)) whose expression remained elevated until 60 hours (panel (ii)). Pretreatment of iDCs with resveratrol prevented RAGE upregulation in response to AGE-albumin at all-time points investigated (panel (ii)). Results are expressed as mean fluorescence intensity (MFI) (means ± SD, n = 3). *P < 0.001. † P < 0.05. (b) Western blotting analysis of RAGE expression on DCs. Western blotting followed by densitometric analysis confirmed RAGE downregulation on AGE-albumin-stimulated DCs by resveratrol (means ± SD, n = 3). ∗† P < 0.001.