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. 2013 Jul 9;2013:574029. doi: 10.1155/2013/574029

Figure 4.

Figure 4

Cytokine production in dendritic cell (DC) culture supernatants. Five-day human DCs pretreated or not with resveratrol (Resv, 50 μM) were cultured for 18 hours with or without AGE-albumin (AGE, 30 μg/mL). DCs treated with LPS (0.2 μg/mL), albumin (Alb; 30 μg/mL), and resveratrol (Resv; 50 μM) were used as controls. Supernatants were collected after 18 hours to measure IL-12p70, TNF-α, IL-10, and IL-1β by specific ELISA experiments. LPS and AGE-albumin (AGE) triggered a statistically significant upregulation of all cytokine secretions. Pretreatment of iDC with resveratrol prevented the upregulation of all proinflammatory cytokines in response to AGE-albumin. Results are expressed as means ± SD of four independent experiments (IL-12p70: *P < 0.01, †‡ P < 0.001; TNF-α and IL-1β: ∗†‡ P < 0.001, § P < 0.05; IL-10: ∗† P < 0.001).