Figure 1.

Modulation of S100B production does not affect proliferation of GL261 glioma cells in vitro. Cells were stably transected with plasmids that carried either S100B siRNA or cDNA to knockdown (S100B^low) or enhance (S100B^high) S100B expression, respectively. Cells transfected with bank plasmid (S100B^wt) were used as control. S100B production by each cell line was measured by A, Western blot and B, ELISA. In vitro proliferate rates of S100B^low and S100B^high cells were similar to control cells as measured by C, cell number/plate or, D, BrdU uptake. Experimental results are representative of at least two separate experiments (n=3, ±SD). * : p < 0.05.