S100B stimulates RAGE expression. A, Western analysis demonstrating in vitro upregulation of full-length (FL)-RAGE in the S100Bhigh cells. B, In vivo FL-RAGE expression was measured in two-week old i.c. gliomas by Western (top) and RT-PCR (bottom, n=3 mice/group), NB: normal brain. C, ONO-2506 (30 mg/kg/day for two weeks) inhibited the expression of S100B in S100Bwt gliomas. D, RAGE expression (red stain) in tumor (T) and tumor periphery in three-week-old (S100Blow) and two-week-old (S100wt and S100Bhigh) i.c. gliomas. E, RAGE expression (red events) by tumor-associated leukocytes was measured in GL261 gliomas two weeks after i.c. implantation. Microglia (CD11bhigh CD45low) RAGE expression was significantly lower in S100Blow gliomas. (n= 3 mice /group, ±SD) * : p < 0.05. All results are representative of three separate experiments.