Fig. 3.

Sox11b expression is elevated in kdrl⁺ cells isolated from avascular mutant embryos. (A) Quantitative RT-PCR analyses confirmed the up-regulated expression of sox11b in endothelial cells of avascular mutant embryos. Two additional transcription factor, klf5l and zfhx4, were used as positive controls. (B) Temporal expression change of sox11b expression in endothelial cells. (C) In situ hybridization of sox11b at 24hpf wild-type embryo. In addition to neural tube and somite, axial vessels express sox11b. Anterior somite, axial vessel, and caudal vein are indicated by the yellow arrow, asterisk, and black arrow, respectively. (D) Effects of BMP signaling on sox11b expression. A decreased activity of BMP signaling significantly reduces the expression of sox11b in endothelial cells.