Table 3.
Effect of protease inhibitors, metal ions, and solvents on proteolytic activity of B. subtilis MA21.
| Substance | Final concentration | Relative activity (%) | Final concentration | Relative activity (%) |
|---|---|---|---|---|
| Control | 0 | 100 | 0 | 100 |
| β-mercaptoethanol | 5 mM | 48 | 1 mM | 70 |
| PMSF | 5 mM | 0 | 1 mM | 0 |
| EDTA | 5 mM | 13 | 1 mM | 96 |
| SDS | 0.5% | 25 | 0.1% | 99 |
| ZnCl2 | 10 mM | 92 | 5 mM | 93 |
| MgCl2 | 10 mM | 22 | 5 mM | 98 |
| CuSO4 | 10 mM | 23 | 5 mM | 73 |
| Urea | 10 mM | 24 | 5 mM | 52 |
| HgCl2 | 10 mM | 27 | 5 mM | 37 |
| CaCl2 | 10 mM | 65 | 5 mM | 142 |
| BaCl2 | 10 mM | 29 | 5 mM | 69 |
| Guanidin HCl | 10 mM | 42 | 5 mM | 49 |
| MnCl2 | 10 mM | 64 | 5 mM | 67 |
| Methanol | 1% | 92 | 0.5% | 99 |
| Ethanol | 1% | 92 | 0.5% | 97 |
| DMSO | 1% | 78 | 0.5% | 96 |
| Isopropanol | 1% | 98 | 0.5% | 101 |
| Tween 20 | 1% | 78 | 0.5% | 86 |
| Triton X100 | 1% | 57 | 0.5% | 133 |