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. Author manuscript; available in PMC: 2013 Jul 29.
Published in final edited form as: Am J Vet Res. 2010 Aug;71(8):925–933. doi: 10.2460/ajvr.71.8.925

Figure 2.

Figure 2

Standard curves for 2 types of real-time qPCR assays of EEHV1 MDBP plasmid diluted in E maximus DNA. Each standard curve is representative of 3 independent experiments. The assay performed with SYBR green detection (diamonds) yielded the following reaction parameters: regression equation, y = −3.338x + 36.57; R2 = 0.999; assay efficiency, 99.4%. The assay performed with hydrolysis probe (circles) yielded the following reaction parameters: y = −3.376x + 38.99; R2 = 0.999; assay efficiency, 97.8%.