Table 2.
Results of detection of EEHV1 DNA in trunk-wash samples obtained from 5 captive Asian elephants of the same herd, from June through August 2009.
| Date of test (mo/d) |
|||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Elephant No. |
6/22 | 6/29 | 7/6 | 7/13 | 7/20 | 7/27 | 8/3 | 8/10 | 8/14 | 8/17 | 8/19 | 8/21 | 8/24 | 8/26 | 8/28 | 9/3 | 9/8 | 9/10 | 9/14 | 9/21 | 9/28 |
| 1 | − | − | − | NR | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − |
| 2 | + | + | + | + | + | +* | +† | +† | +† | + | + | + | + | NR | − | − | − | − | − | − | − |
| 3 | − | +† | + | + | +† | +† | − | NR | NT | + | NT | NT | + | + | − | − | + | + | + | + | + |
| 4 | − | − | − | +† | − | − | − | − | NT | + | NT | NT | − | NT | − | NT | − | NT | − | − | − |
| 5 | − | − | − | − | − | − | − | − | NT | − | NT | NT | − | NT | − | NT | − | NT | − | − | − |
*
Positive result obtained with SYBR green methods (CT = 33.9).
†
Gene subtyping analysis was available on selected positive samples (see tables 4).
− = Test result indicated EEHV1 was absent, with 99.9% confidence. + = Test result indicated EEHV1 was present, with 99.9% confidence in all wells tested in triplicate. NR = No result from assay because of lack of amplification of internal positive control sample (IFN-γ). NT = No test performed on this date.
Test triplicates with a mean CT ≥ 36.2 were deemed negative. Mean positive test triplicate CT values ranged from 26.6 to 36.2.