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. Author manuscript; available in PMC: 2013 Jul 29.
Published in final edited form as: Exp Cell Res. 2011 Apr 2;317(11):1548–1566. doi: 10.1016/j.yexcr.2011.03.012

Fig. 9. Modulation of TRAIL-induced apoptosis in HSF using inhibitory mAbs and low molecular inhibitors of cell signaling pathways.

Fig. 9

A, B. Effects of suppression IKKβ-NF-κB by BMS-345541 (10 μM) and JAK2-STAT3 by AG490 (50 μM) on 2×NF-κB-Luc and 3×STAT3-Luc reporter activities, respectively, in transiently transfected HSF. C. Effects of suppression IKKβ-NF-κB by BMS-345541 (10 μM) and JAK2-STAT3 by AG490 (50 μM) on TRAIL-induced apoptosis. Apoptotic levels (% cells in pre-G1/G0) were determined 48 h after treatment using PI staining and FACS analysis. Error bars represent mean ± S.D. (Student's t test, p< 0.05). D. Effect of IKKβ-NF-κB inhibitor, BMS-345541 (10 μM), on relative increase in IL6 secretion in ρ+ and ρ0 HSF 48 h after exposure to ionizing irradiation. E. Effects of blockage of IL6- or IL8-dependent signaling with anti-IL6 mAb (5 μg/ml) or anti-IL8 mAb (5 μg/ml), respectively, added into the cell media on TRAIL-induced apoptosis in ρ+ and ρ0 HSF. Cells were additionally pretreated with 0.5 Gy α-irradiation (the right panel). Error bars represent mean ± S.D. (Student's t test, p< 0.05).