FIG. 4.

Physiological and material elastic moduli after 21 days culture. A tensile testing rig (A) was used to determine the physiological moduli, shown as region 1 in (B), and the material moduli shown as region 2 in (B). Physiological moduli (C) were determined by finding the slope of the linear region between 10.6 kPa (80 mmHg) and 16.0 kPa (120 mmHg). Material moduli values (D) are highest in controlled oxygen gradient conditions. Asterisks, hash marks, and plus signs (*, #, and+) indicate that the Tukey's test found the results to be statistically significant between two groups at p<0.05. n=6.