Table 2.
Effect of solvent and detergents on protease activity fromBacillus subtilisstrain VV
| Effect of solvent | Effect of detergents | |||
|---|---|---|---|---|
| Solvent (1%) | Residual activity (%) | Detergent (1%) | Relative activity (%) | |
| after 1 h | after 24 h | |||
| n-Butanol | 66.3 ± 4 | Sun light | 113 ± 7 | 50 ± 4 |
| Toluene | 77.2 ± 4 | mr. White | 84 ± 5 | 14 ± 1 |
| Methanol | 102.2 ± 3 | Henko | 99 ± 4 | 30 ± 5 |
| Acetone | 80.5 ± 3 | Ujala | 110 ± 5 | 19 ± 3 |
| Acetonitrile | 99.2 ± 8 | Tide+ | 85 ± 6 | 81 ± 5 |
| Benzene | 82.6 ± 6 | Surf excel | 93 ± 5 | 31 ± 4 |
| Ethanol | 92.5 ± 6 | Aircel | 13 ± 2 | 0 |
The protease was pre-incubated with solvent and surfactants at pre-determined time and the remaining activity was measured using the standard protease assay. Residual and relative enzyme activity was determined as percentage of control with no additions.