Anti-inflammatory effects of 3,4,6-O-Bu3GlcNAc exposure on chondrocytes in monolayer culture. (A) Time line of monolayer gene expression study. (B) Expression levels of genes related to osteoarthritis and NFκB signaling in the presence of 50 μM of 3,4,6-O-Bu3GlcNAc with or without interleukin-1β (IL-1β). Data are presented as averages and SEM of 3 independent samples (*p<0.05, **p<0.01, ***p<0.001 vs. the negative control with respective IL-1β concentrations).