FIG. 3.

IFN induction after nucleofection of mouse fibroblasts with plasmid DNAs. (A) The double modified destination vector, phGfΔGΔR, was introduced into NIH3T3 cells by nucleofection and the levels of IFNβ mRNA at 5 h post-transfection were quantified by qRT-PCR. Data represent the values of three experiments. (B) NIH3T3 cells were nucleofected with VP2-EGFP-N1, VP3-EGFP-N1, or EGFP-N1, and at 5 h post-transfection, the supernatants of growing cells were used for detection of IFNβ levels by ELISA. Data represent the values of two independent experiments. For all experiments (A, B) mock-transfected cells were used as a negative and cells transfected with phGfΔG plasmid as a positive control.