(A) Each experimental heat pain trial included a cue (indicating either High or Low pain), an anticipation period (black crosshair), a painful stimulus (red crosshair), a resting period (black crosshair), a pain rating scale (0–20), and an inter-stimulus interval (black crosshair). (B) The brain networks associated with experience of pain (contrast: high pain (HP) > low pain (LP)) across three functional runs; (C) Brain regions involved in the effects of predictive cue modulation during anticipation of pain (low-cue high pain (LC) >high-cue high pain(HC)); (D) Brain regions involved in cue modulation effects during pain administration (LC > HC).