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. 2001 May 1;29(9):1982–1988. doi: 10.1093/nar/29.9.1982

Figure 3.

Figure 3

Gene transfer by GST–Z2 and Lipofectamine. GST–Z2 was incubated with BAC 269-21 DNA and then diluted in SFM containing Lipofectamine. The gene transfer complexes were added to subconfluent CHOC 400 cells and expression of EGFP from bicistronic Zipro1–IRES–EGFP mRNA was evaluated by confocal microscopy 4 days later. (A) Untransfected cells. (B) Cells transfected with a CMV-GFP expression plasmid (pK7-GFP) with Lipofectamine as a control for GFP expression. (C) Cells transfected with BAC 269-21 DNA using Lipofectamine and Plus reagent. (D) Cells transfected with BAC 269-21 using GST–Z2 and Lipofectamine. (E) Phase contrast image of cells in (D) at higher magnification. (F) GFP expression in the field of cells shown in (E). Note condensation and extrusion of GFP-positive cells from the culture dish, a morphology consistent with apoptosis.