Skip to main content
. 2001 May 1;29(9):1982–1988. doi: 10.1093/nar/29.9.1982

Figure 5.

Figure 5

Analysis of TP53 BACs by SSCP and direct DNA sequencing. Purified DNA from the indicated TP53 BACs was analyzed by SSCP and direct DNA sequencing. (A) Shown is the SSCP analysis for exons 2–5. Exon 10 appears wild-type in all BACs by SSCP but was not sequenced. Arrows indicate altered migration of PCR products for exons with nucleotide polymorphisms (exons 2–4) or mutations (exon 5). (B) Summary of analysis of TP53 BACs by SSCP and direct DNA sequencing. SFT indicates band shifts that were correlated with sequence polymorphisms or mutations as detected by direct DNA sequencing. BAC 66H22 appears to contain the full-length, wild-type human TP53 gene.