Figure 2.

Characteristics of FACS CD44⁻ fraction isolated from lysed bone marrow. (A): Viable nucleated cells obtained from lysed bone marrow from healthy donors. (B): Differential cell count compared with TNCs. (C): Number of sorted CD45⁻CD73⁺CD90⁺CD105⁺CD44⁻ cells obtained from CD34/CD133-depleted bone marrow. (D): Percentage of CD45⁻CD73⁺CD90⁺CD105⁺CD44⁻ cells normalized to TNCs. (E): Growth potential of CD45⁻CD73⁺CD90⁺CD105⁺CD44⁻ cells over 21 days. Data are presented as mean ± SEM, n = 5. Differential cell counts were performed using an automated hematology analyzer (Beckman Coulter). Abbreviations: FACS, fluorescence-activated cell sorting; TNC, total nucleated cells.