Figure 7. Effect of the rs9939609 FTO genotype on GHRL mRNA abundance, immunodepletion of m⁶A-methylated GHRL mRNA, and RPS14 mRNA in peripheral blood cells.

(A) GHRL mRNA abundance in peripheral blood cells from TT (blue bars) and AA (red bars) study subjects. Effect of the FTO genotype on immunodepletion of m⁶A-methylated (B) GHRL mRNA and (C) RPS14 mRNA abundance from total cellular mRNA in TT and AA study subjects. mRNA abundance was determined by qPCR and calculated relative to the input sample, with transcripts normalized to the amount of GUSB mRNA (a non-m⁶A–containing mRNA). (D) FTO mRNA abundance in peripheral blood cells. Data are presented as the mean ± SEM; n = 10 per genotype. **P < 0.01. Difference between normalized TT unbound and normalized AA unbound; ^###P < 0.001.