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. 2013 Jul 1;123(8):3211–3230. doi: 10.1172/JCI65521

Figure 3. APEX1 upregulates Jagged1 transcription.

Figure 3

(A) Number of common and unique APEX1 target genes in migration, proliferation, and angiogenesis of DLD1 and SW480 cells. (B) Heat map showing 4 common genes, including JAG1 (arrow), involved in the 3 tumorigenic processes in A. (C) Quantitative real-time RT-PCR for relative JAG1 mRNA from DLD1 cells transfected with control or APEX1 siRNA and from SW480 cells transfected with control or APEX1 expression vector. (D) Western blot analysis of Jagged1 expression in the indicated cells. (E) Number of common and unique APEX1 target genes involved in migration and in proliferation and differentiation of GM00637 cells. (F) Heat map showing 7 common genes, including JAG1 (arrow), involved in the 2 biological processes in E. (G) Relative expression of JAG1 mRNA in GM00637 cells transfected with control or APEX1 expression vector. (H) Western blot analysis of Jagged1 expression in the indicated GM00637 cells. Results in C and G are mean ± SD (n = 3). **P < 0.01.