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. 2013 Jul 1;123(8):3211–3230. doi: 10.1172/JCI65521

Figure 6. Jagged1 knockdown attenuates tumorigenicity in APEX1-overexpressed SW480 and HT29 cells.

Figure 6

SW480-APEX1 and HT29-APEX1 cells were transfected with control or Jagged1 siRNA. (A) Cell growth. Lysates of cells were examined for Jagged1 and APEX1 expression by Western blot analysis. (B) Colony formation in soft agar after 14 days of culture. (C) Cell migration and invasion. Data represent the average cell numbers from 5 viewing fields. (D) Tube formation assays were performed by culturing HUVECs in tube formation with supernatants from the indicated cells. Results in A–D are mean ± SD (n = 3). **P < 0.01. Scale bars: 80 μm (B and D); 40 μm (C).