Figure 2. Rapamycin treatment had limited effects on aging-associated neurobehavioral phenotypes.

Basic neurological functions were assessed in the 16-month (young control, n = 10; vehicle, n = 18; rapamycin, n = 20) and 25-month (young control, n = 10; vehicle, n = 11; rapamycin, n = 16) cohorts. (A and B) Latency to fall in the context of a motor coordination test on the accelerating rotarod. (C and D) Latency to first and second reaction on a hot plate test performed to assess nociceptive functions. (E and F) Exploratory activity, as examined in an open field assay. (E) Distance traveled. (F) Number of rearings. (G and H) Effects of rapamycin on exploratory activity in young mice (vehicle, n = 9; rapamycin, n = 8). Whisker plots display minimum, 25th percentile, median, 75th percentile, and maximum. P values and fit coefficients with 95% confidence intervals are shown; statistically significant differences (P < 0.05) are denoted by bold font.